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KMID : 0382420020280010021
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Korean Journal of Environmental Health Society
2002 Volume.28 No. 1 p.21 ~ p.30
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Study on measurement of DNA adducts formed in liver cells and bladder epithelial cells of rats exposed dichlorobenzidine (DCB) by ³²P-postlabeling and GC/MS-SIM method
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Lee Jin-Heon
Shin Ho-Sang
Jang Mi-Seon
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Abstract
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To identify and evaluate the dichlorobenzidine(DCB)-DNA adducts in liver cell and bladder epithelial cells by ³²P-postlabeling and GC/MS-SlM, we orally exposed the dichlorobenzidine(2Omg/kh body wL/day) to male Sprague-Dawley rats(l85±10g) for 14 days.
Two kinds of DCB-DNA adduct(A l and A2)were found at the same site of thin layer chromatogram of ³²P. postlabeling method ln liver cells and bladder eplthelial cells. ln liver cells, relative adduct labeling(RAL)×10¹² of DCB-DNA adduct Al were34.l±3.7I and 69.9±5.O2, that of adduct A2 were 74.l±l0.l and I05.I±l0.I on I0 and l4 days after treatment, respectivel. And in bladder epithelia cells. RAL×10¹² of DCB-DNA adducl Al were 5.92±I.60 and l5.9±1.3l. that of adduct A2 were 9.8l±2.8l and 22.8±1.79 on 10 and l4 days after treatment, respectively.
DCB metabolites formed DNA adducts were monoacetyl-dichlorobenzidine(acDCB) and diacetyl- dichlorobenzidine(di-acDCB), which was identify by gas chromatography/mass spectrometry-scan ionization mode(GC/MS-SIM). after hydrolysis of DCB-DNA adducts isolated from live cells and bladder epithelial cells. The base peak of acDCB were 252 and 294 m/z, and that of di-acDCB were 252, 294 and 336 m/z.
ln conclusion, the exposed DCB formed two kinds of DCB-DNA adduct, the proximate materials of that were acDCB and di-acDCB in liver and bladder epithelial cells. And the above GC/MS-SIM method was found the DCB-DNA adducts could be monitoring by gas chromatography.
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